Meristem culture of Prunus avium cvs. “Siyah-e-Mashhad” and “Takdane”

Mahbube Zamanipour1, Ebrahim Ganji Moghaddam2*, Ali Tehranifar3, Bahram Abedi4

DOI: 10.29252/azarinj.003

ABSTRACT

The present trial aimed to evaluate the effect of three media including WPM, MS and modified QL and three different hormone combinations (0, 0.5 and 1 mgL-1 BAP) with 0.1 mgL-1 GA3 and 0.1 mgL-1 IBA on regeneration of two sweet cherry cultivars cvs “Siyah-e-Mashhad” and “Takdane”. All experiments were arranged in completely randomized designed. Each treatment contained three replicates. The meristem explants were introduced in culture media supplemented with different hormonal combinations and maintained in a growth room at a 16h photoperiod (36 μmol.m-2.s-1), 25 ± 1ºC. After six weeks, Survival, necrosis and contamination percent with the number of leaf were studied. The results showed that highest survival rate was in modified QL medium complemented with 1 mgL-1 BAP and the least one was in MS medium without BAP treatments for both cultivars. Necrosis rate was high in MS (100-83.3%) in control for “Takdane” and “Siyah-e-Mashhad” cultivars, respectively that don’t have any differences with 0.5 mgL-1 BAP. The most Leaf number (12) observed in modified QL medium supplemented with 1 mgL-1 BAP in “Takdane” cultivar and the least one (2) was in MS medium without BAP application (control) in “Siyah-e-Mashhad” cultivar.

Keywords:Browning Rate, Contamination Rate, Leaf Number, Meristem Culture, Survival Rate, Sweet Cherry

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References

Bonga J.M. Van Adercas P. (2002) Tissue culture of woody plants. Translated by Arvin MJ Shahid Bahonar University Publications. Kerman, Iran.

Bhatia P. Ashwath N. (2008) Improving the quality of in vitro culture shoots of tomato. Biotechnology. 7: 188–193. doi: 10.3923/biotech.2008.188.193.

Chakrabarty D. Hahn E.J. Yoon Y.J. Paek. K.Y. (2003) Micropropagation of apple rootstock M.9 EMLA using bioreactor. Journal of Horticultural Science and Biotechnology, 65(1): 605-609.

Chien-Ying K. Al-Abdulkarim A.M. Al-Jowid S.M. Al-Baiz A. (2009) An effective disinfection protocol for plant regeneration from shoot tip cultures of strawberry. African Journal of Biotechnology, 8: 2611–2615.

Clapa D.F. (2007). Tissue culture and ex vitro acclimation of Rhododendron sp., Buletinul USAMV-CN.

Erbenova M. Papr štein F. Sedl Ak J. (2001) In vitro propagation of dwarfed rootstocks for sweet cherry. Acta Horticulturae, 560: 477–480.

FAO. (2017) On-line crop database ECOCROP. URL: http://ecocrop.fao.org/ecocrop/srv/en/cropSearchForm.

García-Gonzáles R. Quiroz K. Caligari P.D.S. Carrasco B. (2010). Plant tissue culture: current status, opportunities and challenges. Ciencia e Investigación Agraria. 37: 5–30. doi: 10.4067/S0718-16202010000300001.

Husain M.K. Anis M. (2009) Rapid in vitro multiplication of Melia azedarach L. (a multipurpose woody tree) Acta Physiologiae Plantarum, 31:765–772. doi: 10.1007/s11738-009-0290-7.

Isac V. Coman T. Marinescu L. Isac M. Teodorescu A. Popescu A. Coman M. Plopa C. (2010) Achievements and Trends in the Use of Tissue Culture for the Mass Propagation of Fruit Plants and Germplasm Preservation at the Research Institute for Fruit Growing, Pitesti, Roman. Romanian Biotechnological Letters.

Jakab I. Zs. Pamfil D. Clapa D. Fira A. (2008) In vitro regeneration and meristem culture of Prunus domestica cv. Bulletin UASVM, Horticulture, 65 (1): 126-131.

Lioyd G. McCown B. (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifoliaby use of shoot-tip culture. Combined Proceeding, International Plant Propagators Society, 30: 421-427.

Mert C. Soylu S. (2010) Shoot location and collection time effects on meristem tip culture of some apple rootstocks. Pakistan Journal of Botany, 42 (1): 549-557.

Mozafari A. Bahramnejad. B. (2010) The effects of medium and hormones mixture on the morphological changes of Fragaria annanasa by meristem culture. Life Technology, 9(2): 17-29.

Murashige T. Skoog F. (1962) A revised medium for rapid growth and bioassay with tobacco culture. Journal of Plant Physiology, 15: 473-497. http://dx.doi.org/10.1111/j1399-3054

Öztürk Ö. (2004) Micropropagation of some apple clonal rootstocks. MS Thesis. Uludag University, Institute Science, Bursa. 42 .

Perez-Sanchez R. Gomez-Sanchez M.A. Morales-Corts M.R. (2010) Description and quality evaluation of sweet cherries cultured in spain. Journal of Food Quality, 33: 490-506. DOI: 10.1111/j.1745-4557.2010.00339.x

Pérez-Tornero O. Burgos L. (2007) Apricot micropropagation. In: Jain, S.M. and Haggman (eds.), Protocols for micropropagation of woody trees and fruits, 267-278.

Quoirin M. and Lepoivre P. (1977) Etude de milieux. Contaminants of adaptes aux cultures in vitro de Prunus. Acta Horticulturae, 78: 437-442.

Quiroz K.A. Berríos M. Carrasco B. Retamales J.B. Caligari P.D.S. García-Gonzáles R. (2017) Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.). Journal of Biological Research. 50: 20. doi: 10.1186/s40659-017-0125-8.

Salami S. Ebadi H. Zamani Z. (2005) The effects of explant times, apex size and culture medium on the initiation phase of meristem culture in Vitis vinifera cvs. Bidane and Shahrudi. Investigation in Agricultural and Horticultural Science, 67: 72-81.

Snedecor G.M. Cochran W.G. (1986) Statistical Methods. 9 Ed., The Iowa State University, Press. Th. Amer. Iowa, U.S.A. 07 P.

Soliman H. (2012). In vitro propagation of Apricot (Prunus armeniaca L.) and assessment of genetic stability of micropropagated plants using RAPD analysis. World Applied Sciences Journal, 19(5): 674-687. http://dx.doi.org/105829/idosi.wasj.2012.19.5.2770.

Tioleneve V.M. (1993) Regeneration of Front Crops from Somatic Tissues, 1st edition, Pushino 173.